![]() ![]() Thus, the first two dilution steps involve taking 10ml of solution and adding 90ml of water, whereas the third step involve staking 10ml of solution and adding 70ml of water. This can be easily decomposed into two tenfold dilutions and one eightfold dilution. Rather than taking 10ml of solution and adding 90ml of water to give a tenfold dilution, 20ml of solution is taken and 80ml of water adding to give a fivefold dilution.Į) To give a concentration of 125 cells/ml requires a dilution of 800 times. ![]() Thus, to give the required concentration involves taking 20ml of solution and adding 30ml of water, and then taking 10ml of solution and adding 90ml of water, before repeating both of these steps.ĭ) To achieve a 20 cells/ml concentration is similar to that of the 10 cells/ml dilution, except that the final step is different. Thus only three tenfold dilutions occur, and so the final concentration is 100 cells/ml as opposed to 10 cells/ml.Ĭ) To give a concentration of 160 cells/ml requires a 625 times dilution, which can be decomposed into a two 2.5x dilutions and two 10x dilutions. Thus, 10ml of solution should be transferred each time, and 90ml of water added to it.ī) To give a concentration of 100 cells/ml, the same process should be repeated as in a) except that the final addition of water should not occur. Since we have four opportunities to dilute the original solution, this logically requires a tenfold dilution each time. However, only a single solution is provided below:Ī) To achieve a concentration of 10 cells/ml requires a dilution of 10,000 times. ![]() This gives a minimum final concentration of 6 cells/ml.Ģ) It should be noted that for several of the required dilutions, there are more than one possible way to make them. The smallest concentration results from transferring the minimum amount of solution each time (10ml) but adding the maximum amount of water (100ml). 1) It is clear the highest concentration which is achievable is the original concentration of 100000 cells/ml, which results in transferring solution between subsequent beakers but without adding any additional water. ![]()
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